6DLS
PRPP Riboswitch bound to PRPP, thallium acetate soaked structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.220, 91.977, 75.058 |
Unit cell angles | 90.00, 122.80, 90.00 |
Refinement procedure
Resolution | 19.350 - 2.885 |
R-factor | 0.2097 |
Rwork | 0.207 |
R-free | 0.23480 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 19.350 |
High resolution limit [Å] | 2.885 | 2.900 |
Rmerge | 0.098 | 0.826 |
Rpim | 0.050 | 0.454 |
Number of reflections | 19937 | |
<I/σ(I)> | 28.2 | |
Completeness [%] | 97.9 | |
Redundancy | 4.8 | |
CC(1/2) | 0.726 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M NH4-acetate, 0.1 M HEPES-KOH pH 7.4, and 45% 2-methyl-2,4-pentanediol (v/v) |