6DLO

Crystal structure of LRRK2 WD40 domain dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	79.293, 103.590, 112.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.256 - 2.700
R-factor	0.2375
Rwork	0.234
R-free	0.27530
RMSD bond length	0.002
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.070	2.730
High resolution limit [Å]	2.610	2.610
Number of reflections	28401
<I/σ(I)>	14.6
Completeness [%]	99.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Tris-HCl at pH 8.5, 1 M LiCl, 16% polyethylene glycol (PEG) 6000, and 10% additive of 30% galactose