6DLK
Crystal structure of DNA polymerase III subunit beta from Rickettsia rickettsii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.090, 97.570, 115.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.649 - 2.000 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tsz |
RMSD bond length | 0.015 |
RMSD bond angle | 1.201 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX ((1.14_3211)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.649 | 36.649 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.056 | 0.019 | 0.584 |
Rmeas | 0.061 | 0.021 | 0.644 |
Total number of observations | 368066 | ||
Number of reflections | 60964 | 737 | 4462 |
<I/σ(I)> | 22.18 | 58.93 | 3.1 |
Completeness [%] | 99.6 | 95 | 100 |
Redundancy | 6.037 | 5.271 | 5.648 |
CC(1/2) | 0.999 | 1.000 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 290 | Rigaku Reagents Top96 G6: (0.5 M Ammonium Sulfate, 0.1 M Sodium Citrate:Citric Acid pH 5.6, 1 M Lithium Sulfate) mixed 1:1 with 20 mg/mL protein in sitting drop vapor diffusion. Tray id 291006g9, crystal id qtv9-3 |