6DLH
Endo-fucoidan hydrolase MfFcnA4 from glycoside hydrolase family 107
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97531 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 156.600, 156.600, 84.571 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.230 - 2.200 |
| R-factor | 0.2192 |
| Rwork | 0.217 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FcnA4_build1.pdb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.007 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.870 | 39.870 | 2.260 |
| High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
| Rmerge | 0.126 | 0.029 | |
| Rmeas | 0.132 | 0.030 | 0.963 |
| Rpim | 0.040 | 0.010 | 0.296 |
| Total number of observations | 648258 | ||
| Number of reflections | 60747 | 732 | 4478 |
| <I/σ(I)> | 14.9 | ||
| Completeness [%] | 100.0 | 98 | 100 |
| Redundancy | 10.7 | 10.1 | 10.5 |
| CC(1/2) | 0.998 | 0.999 | 0.924 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M NaCl, 1.4 M (NH4)SO4, sodium acetate:acetic acid, (pH 4.5) |
