6DLH

Endo-fucoidan hydrolase MfFcnA4 from glycoside hydrolase family 107

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97531
Spacegroup name	P 31 2 1
Unit cell lengths	156.600, 156.600, 84.571
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.230 - 2.200
R-factor	0.2192
Rwork	0.217
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FcnA4_build1.pdb
RMSD bond length	0.003
RMSD bond angle	1.007
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.870	39.870	2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.126	0.029
Rmeas	0.132	0.030	0.963
Rpim	0.040	0.010	0.296
Total number of observations	648258
Number of reflections	60747	732	4478
<I/σ(I)>	14.9
Completeness [%]	100.0	98	100
Redundancy	10.7	10.1	10.5
CC(1/2)	0.998	0.999	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M NaCl, 1.4 M (NH4)SO4, sodium acetate:acetic acid, (pH 4.5)