6DLA

Crystal structures of an influenza A hemagglutinin antibody Fab CH65:7969d2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9775
Spacegroup name	P 21 21 21
Unit cell lengths	57.378, 67.238, 130.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.646 - 2.004
R-factor	0.1791
Rwork	0.178
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wuk
RMSD bond length	0.007
RMSD bond angle	0.836
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.101	0.391
Number of reflections	34481
<I/σ(I)>	10.4
Completeness [%]	99.5
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.16	AmSO4 1.8-2.2M, Bis-Tris 100 mM pH 6.5