6DKU

Crystal structure of Myotis VP35 mutant of interferon inhibitory domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.77110
Spacegroup name	P 32 2 1
Unit cell lengths	78.126, 78.126, 43.471
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.063 - 2.600
R-factor	0.1733
Rwork	0.171
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l25
RMSD bond length	0.005
RMSD bond angle	0.715
Data reduction software	XDS (Oct 15,2015)
Data scaling software	autoPROC (1.1.7)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.063	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.087	1.420
Number of reflections	4730	409
<I/σ(I)>	11.97
Completeness [%]	95.1
Redundancy	6.8
CC(1/2)	0.998	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M HEPES pH 7.5, 25% (w/v) PEG 3350