6DKT

Crystal structure of Arginase from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	77.140, 128.550, 82.020
Unit cell angles	90.00, 112.69, 90.00

Refinement procedure

Resolution	47.701 - 2.080
R-factor	0.2048
Rwork	0.204
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g3h
RMSD bond length	0.005
RMSD bond angle	0.733
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.701	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.060	0.280
Rmeas	0.085	0.396
Number of reflections	88598	8819
<I/σ(I)>	6.26	1.92
Completeness [%]	100.0	100
Redundancy	1.9	1.8
CC(1/2)	0.993	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM Bis Tris, 14.5% PEG3350, 5% PGA