6DKD
Yeast Ddi2 Cyanamide Hydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0246 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 264.643, 264.643, 119.304 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.768 - 3.000 |
| R-factor | 0.1951 |
| Rwork | 0.194 |
| R-free | 0.22050 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6dk9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.947 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.092 | 0.568 |
| Number of reflections | 92614 | 4667 |
| <I/σ(I)> | 20.9 | 2.7 |
| Completeness [%] | 96.5 | 97.8 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 1.1-1.3 M Ammonium Sulphate 0.2 M Arginine 0.1 M N-morpholino ethane sulfonate pH 5.8 |
