6DKC
Yeast Ddi2 Cyanamide Hydratase, T157V mutant, apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0246 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 263.613, 263.613, 119.026 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.300 - 2.900 |
| R-factor | 0.1932 |
| Rwork | 0.193 |
| R-free | 0.21540 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6dk9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.858 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.105 | 0.695 |
| Number of reflections | 102026 | 5127 |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 97.0 | 98.8 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 1.1-1.3 M Ammonium Sulfate 0.2 M arginine 0.1 M N-morpholino sulfonate, pH 5.8 |
