6DK2

Bacteroidetes AC2a SusD-like

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-15
Detector	RAYONIX MX-300
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	101.703, 140.269, 186.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.930 - 2.020
R-factor	0.1528
Rwork	0.152
R-free	0.17610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j5u
RMSD bond length	0.004
RMSD bond angle	0.921
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.930	2.094
High resolution limit [Å]	2.020	2.020
Rmerge	0.085	0.521
Rmeas	0.093
Number of reflections	172008	17235
<I/σ(I)>	11.13	2.27
Completeness [%]	99.7	99.99
Redundancy	6.2	6.2
CC(1/2)	0.997	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.5 M ammonium sulphate, 50 mM Tris-HCl pH 7.5