6DIN
High resolutionstructure of apo dTDP-4-dehydrorhamnose 3,5-epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.854, 58.854, 160.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.616 - 1.800 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.20590 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 6.150 | 1.800 |
| Rmerge | 0.084 | 0.061 | |
| Total number of observations | 303807 | ||
| Number of reflections | 27242 | 807 | 665 |
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 99.9 | 97.6 | 100 |
| Redundancy | 11.2 | 8.8 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | Sodium Chloride, bis-Tris, Ammonium sulfate |
