6DIC
D276G DNA polymerase beta substrate complex with templating cytosine and incoming Fapy-dGTP analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-16 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.800, 80.400, 55.500 |
| Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
| Resolution | 20.657 - 1.992 |
| R-factor | 0.1809 |
| Rwork | 0.175 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.062 | 0.039 | 0.368 |
| Total number of observations | 95086 | ||
| Number of reflections | 27639 | 2844 | 2557 |
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 96.4 | 97.1 | 89.8 |
| Redundancy | 3.4 | 3.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50mM Imidazole, 350mM Sodium Acetate, 16% Peg3350 |
