6DHZ

Crystallographic octamer of a metal-free RIDC1 variant bearing two disulfide bonded cysteines

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 2 2
Unit cell lengths	78.550, 78.550, 168.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.550 - 2.800
R-factor	0.21128
Rwork	0.208
R-free	0.27665
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iq6
RMSD bond length	0.013
RMSD bond angle	1.589
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.550	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	13669	1915
<I/σ(I)>	16.8
Completeness [%]	99.7	99.2
Redundancy	9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	15% 2-methyl-2,4-pentane diol (v/v), 33.3 mM Bis-Tris (pH 6.5), 66.7 mM CaCl2