6DHY
Crystallogrpahic tetramer of Zn-bound RIDC1 variant bearing two disulfide bonded cysteines
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 |
| Unit cell lengths | 52.510, 52.510, 255.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.840 - 2.220 |
| R-factor | 0.23016 |
| Rwork | 0.229 |
| R-free | 0.25848 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iq6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.498 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.880 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 19755 | |
| <I/σ(I)> | 23.2 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 8% PEG 1900 MME (w/v), 40 mM CaCl2, 33.3 mM Tris (pH 7.5) |
