6DHX

Structure of TipC2 from Streptococcus intermedius B196

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	159.740, 54.520, 104.430
Unit cell angles	90.00, 108.05, 90.00

Refinement procedure

Resolution	33.600 - 1.750
R-factor	0.1705
Rwork	0.170
R-free	0.19430
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.221
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.600	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	85866
<I/σ(I)>	21.1
Completeness [%]	99.2
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	100mM Tris-HCl pH 8.5, 25% w/v PEG 3350