6DHS

Structure of hnRNP H qRRM1,2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9787
Spacegroup name	P 64 2 2
Unit cell lengths	204.670, 204.670, 123.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.690 - 3.500
R-factor	0.306
Rwork	0.306
R-free	0.32200
RMSD bond length	0.009
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.560
High resolution limit [Å]	3.500	9.480	3.500
Rmerge	0.148	0.067	0.835
Rmeas	0.150	0.069	0.848
Rpim	0.027	0.013	0.150
Total number of observations	606047
Number of reflections	21278	1104	953
<I/σ(I)>	5.7
Completeness [%]	100.0	99.7	100
Redundancy	30.9	26.5	31.3
CC(1/2)	0.997	0.999	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	30-50% peg 400 and 0.1M phosphate-citrate, pH 4.2