6DHQ

Bovine glutamate dehydrogenase complexed with NADPH, glutamate, and GTP

Replaces:  1HWZReplaces:  3QMU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	123.246, 101.747, 167.852
Unit cell angles	90.00, 102.22, 90.00

Refinement procedure

Resolution	29.552 - 2.300
R-factor	0.2244
Rwork	0.224
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.819
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	108403
<I/σ(I)>	17
Completeness [%]	60.2
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	octyl-b-glucopyranoside, sodium chloride, sodium phosphate, sodium azide, MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K