Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-10 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 121.110, 98.760, 165.640 |
Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
Resolution | 43.888 - 3.300 |
R-factor | 0.2618 |
Rwork | 0.260 |
R-free | 0.30140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.780 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | CNS |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.450 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 60196 | |
<I/σ(I)> | 4.2 | |
Completeness [%] | 96.3 | |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 8% PEG8000, 0.15 M sodium chloride, 5% MPD, 0.1 M triethanolamine-HCl, pH 7.0, 50 mM monosodium glutamate, 2 mM GTP, 2 mM NADPH, VAPOR DIFFUSION, HANGING DROP, temperature 298K |