6DHL
Bovine glutamate dehydrogenase complexed with epicatechin-3-gallate (ECG)
Replaces: 3QMUReplaces: 3MVQExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.120, 433.180, 95.170 |
Unit cell angles | 90.00, 118.74, 90.00 |
Refinement procedure
Resolution | 49.706 - 3.624 |
R-factor | 0.2494 |
Rwork | 0.246 |
R-free | 0.31350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.814 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 72322 | |
<I/σ(I)> | 14.8 | |
Completeness [%] | 95.0 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 5 mg/mL GDH in 0.4 mM ECG, 3 mM NADPH, 20 mM glutamate, 2:1 with reservoir solution (0.9 M sodium chloride, 50 mM TEA-HCl, 5 mM reduced glutathione, 8~9% PEG8000, 1 M 1,6-hexanediol), VAPOR DIFFUSION, HANGING DROP, temperature 295K |