6DHK

Bovine glutamate dehydrogenase complexed with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-01-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	96.382, 172.160, 439.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.935 - 3.500
R-factor	0.2174
Rwork	0.216
R-free	0.25430
RMSD bond length	0.026
RMSD bond angle	2.783
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1633)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.660
High resolution limit [Å]	3.500	3.500
Number of reflections	90105
<I/σ(I)>	14.7
Completeness [%]	94.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	298	sodium phosphate, PEG8000, sodium azide, sodium chloride, MPD, OBG, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K