6DGM
Streptococcus pyogenes phosphoglycerol transferase GacH in complex with sn-glycerol-1-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.650, 82.970, 130.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.532 - 1.490 |
R-factor | 0.154 |
Rwork | 0.153 |
R-free | 0.17540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u9z |
Data reduction software | XDS (VERSION Jan 26, 2018 BUILT=20180126) |
Data scaling software | XSCALE (VERSION Jan 26, 2018 BUILT=20180126) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (dev_3139) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.532 | 39.532 | 1.530 |
High resolution limit [Å] | 1.490 | 6.660 | 1.490 |
Rmerge | 0.080 | 0.020 | 1.143 |
Rmeas | 0.086 | 0.022 | 1.281 |
Number of reflections | 127541 | 1610 | 7144 |
<I/σ(I)> | 14.23 | 55.96 | 1.2 |
Completeness [%] | 96.3 | 97.3 | 73.4 |
Redundancy | 6.488 | 6.067 | 4.963 |
CC(1/2) | 0.999 | 1.000 | 0.496 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.2M calcium acetate, 0.1M MES pH 6.0, 20% PEG8000, 0.01M sn-glycerol-1-phosphate |