6DGC

Crystal structure of the C-terminal catalytic domain of ISC1926 TnpA, an IS607-like serine recombinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97930
Spacegroup name	C 1 2 1
Unit cell lengths	97.080, 212.320, 61.600
Unit cell angles	90.00, 126.68, 90.00

Refinement procedure

Resolution	19.720 - 2.920
R-factor	0.22
Rwork	0.217
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lhk
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.2)
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.720	19.720	2.980
High resolution limit [Å]	2.910	12.990	2.910
Rmerge	0.050	0.029	0.511
Rmeas	0.058	0.033	0.673
Total number of observations	71134
Number of reflections	19649	164	925
<I/σ(I)>	12.85	36.72	1.3
Completeness [%]	88.6	62.1	56.8
Redundancy	3.62	4.091	1.898
CC(1/2)	0.999	0.999	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	Tascimate, PEG3350