6DGB

Crystal structure of the C-terminal catalytic domain of IS1535 TnpA, an IS607-like serine recombinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	52.560, 54.180, 104.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.080 - 2.520
R-factor	0.23
Rwork	0.228
R-free	0.26100
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.080	48.080	2.570
High resolution limit [Å]	2.500	11.190	2.500
Rmerge	0.099	0.058	0.648
Rmeas	0.112	0.067	0.744
Total number of observations	44467
Number of reflections	10275	132	711
<I/σ(I)>	6.48	12.35	1.5
Completeness [%]	95.2	86.8	91.4
Redundancy	4.328	3.705	3.962
CC(1/2)	0.997	0.998	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG3350