6DG4
Structure of the Chaetomium thermophilum Ulp1-like SUMO protease catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.986, 63.595, 83.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.842 - 1.442 |
R-factor | 0.1195 |
Rwork | 0.118 |
R-free | 0.14810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1euv |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.460 |
High resolution limit [Å] | 1.440 | 3.910 | 1.440 |
Rmerge | 0.052 | 0.039 | 0.215 |
Rmeas | 0.056 | 0.043 | 0.234 |
Rpim | 0.021 | 0.017 | 0.091 |
Total number of observations | 310342 | ||
Number of reflections | 44305 | 2401 | 1878 |
<I/σ(I)> | 9.4 | ||
Completeness [%] | 97.9 | 97.2 | 85.2 |
Redundancy | 7 | 6.6 | 6.2 |
CC(1/2) | 0.998 | 0.973 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES, pH 7.0, 2.1 M ammonium sulfate |