6DFW
TCR 8F10 in complex with IAg7-p8G9E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979500 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.094, 102.044, 136.001 |
Unit cell angles | 90.00, 107.83, 90.00 |
Refinement procedure
Resolution | 50.010 - 3.200 |
R-factor | 0.23619 |
Rwork | 0.233 |
R-free | 0.29691 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.124 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 38891 | |
<I/σ(I)> | 7.6 | |
Completeness [%] | 96.9 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 11% PEG 3350, 100mM formate pH7.0 |