6DFV

Mouse diabetogenic TCR 8F10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979500
Spacegroup name	P 1
Unit cell lengths	62.016, 69.691, 69.699
Unit cell angles	111.66, 111.03, 94.86

Refinement procedure

Resolution	48.210 - 1.710
R-factor	0.17389
Rwork	0.171
R-free	0.23113
RMSD bond length	0.015
RMSD bond angle	1.715
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.710	1.710
Number of reflections	91564
<I/σ(I)>	11.62
Completeness [%]	91.5
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	14% PEG 3350, 100mM sodium acetate at pH6.0