6DFS
mouse TCR I.29 in complex with IAg7-p8E9E6ss
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9790 |
Spacegroup name | P 63 |
Unit cell lengths | 269.518, 269.518, 45.639 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.010 - 3.100 |
R-factor | 0.24197 |
Rwork | 0.240 |
R-free | 0.28515 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.096 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.300 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 32259 | |
<I/σ(I)> | 24.4 | |
Completeness [%] | 99.7 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 12% PEG 20000, 100mM bicine pH9.0, 20% ethylene glycol |