6DFQ
mouse diabetogenic TCR I.29
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.107, 117.629, 234.774 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.600 - 2.600 |
R-factor | 0.22153 |
Rwork | 0.220 |
R-free | 0.25253 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.459 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 76175 | |
<I/σ(I)> | 12.4 | |
Completeness [%] | 98.1 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% MPD, 100mM sodium cacodylate at pH6.0, 50mM calcium acetate |