6DFP
Crystal Structure of a Tripartite Toxin Component VCA0883 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.564, 37.491, 73.343 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 24.372 - 1.500 |
| R-factor | 0.1608 |
| Rwork | 0.159 |
| R-free | 0.19510 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.699 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.605 |
| Number of reflections | 54059 | 1960 |
| <I/σ(I)> | 39 | |
| Completeness [%] | 96.8 | 71.2 |
| Redundancy | 3.8 | 2.5 |
| CC(1/2) | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 34.3 % (w/v) PEG 5000 MME, 150 mM AMPD/Tris pH 9.0, 30 mM Sodium Potassium Tartrate |
