6DFD
Crystal structure of CNNM3 cyclic nucleotide-binding homology domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9773 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 101.267, 101.267, 77.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.474 - 1.901 |
| R-factor | 0.2205 |
| Rwork | 0.219 |
| R-free | 0.23870 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.698 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.092 | |
| Number of reflections | 32014 | 3117 |
| <I/σ(I)> | 44.2 | 1.8 |
| Completeness [%] | 99.6 | 99.49 |
| Redundancy | 28.7 | 27.5 |
| CC(1/2) | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.8 M succinic acid |
