6DFD

Crystal structure of CNNM3 cyclic nucleotide-binding homology domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9773
Spacegroup name	P 42 21 2
Unit cell lengths	101.267, 101.267, 77.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.474 - 1.901
R-factor	0.2205
Rwork	0.219
R-free	0.23870
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.698
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.092
Number of reflections	32014	3117
<I/σ(I)>	44.2	1.8
Completeness [%]	99.6	99.49
Redundancy	28.7	27.5
CC(1/2)		0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.8 M succinic acid