6DFD
Crystal structure of CNNM3 cyclic nucleotide-binding homology domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9773 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 101.267, 101.267, 77.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.474 - 1.901 |
R-factor | 0.2205 |
Rwork | 0.219 |
R-free | 0.23870 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.698 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.092 | |
Number of reflections | 32014 | 3117 |
<I/σ(I)> | 44.2 | 1.8 |
Completeness [%] | 99.6 | 99.49 |
Redundancy | 28.7 | 27.5 |
CC(1/2) | 0.662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.8 M succinic acid |