6DFC

Kaiso (ZBTB33) zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS) with a T-to-U substitution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	C 2 2 2
Unit cell lengths	44.240, 184.451, 104.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.020 - 1.850
R-factor	0.1893
Rwork	0.187
R-free	0.20790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6m
RMSD bond length	0.009
RMSD bond angle	1.162
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.020	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.087
Rmeas	0.093
Number of reflections	36573	3333
<I/σ(I)>	17.74	1.84
Completeness [%]	98.2	89.31
Redundancy	9.4	6.8
CC(1/2)	0.996	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.2 M Disodium Tartrate, 20 % PEG 3350