6DFC
Kaiso (ZBTB33) zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS) with a T-to-U substitution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 44.240, 184.451, 104.705 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.020 - 1.850 |
R-factor | 0.1893 |
Rwork | 0.187 |
R-free | 0.20790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f6m |
RMSD bond length | 0.009 |
RMSD bond angle | 1.162 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.020 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.087 | |
Rmeas | 0.093 | |
Number of reflections | 36573 | 3333 |
<I/σ(I)> | 17.74 | 1.84 |
Completeness [%] | 98.2 | 89.31 |
Redundancy | 9.4 | 6.8 |
CC(1/2) | 0.996 | 0.612 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 295 | 0.2 M Disodium Tartrate, 20 % PEG 3350 |