6DFB
Kaiso (ZBTB33) K539A zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 44.467, 184.271, 105.188 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.198 - 1.660 |
R-factor | 0.1811 |
Rwork | 0.179 |
R-free | 0.20160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f6m |
RMSD bond length | 0.009 |
RMSD bond angle | 1.089 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.198 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.074 | |
Rmeas | 0.077 | |
Number of reflections | 50786 | 4731 |
<I/σ(I)> | 14.89 | 2.83 |
Completeness [%] | 98.3 | 92.65 |
Redundancy | 11.2 | 7.9 |
CC(1/2) | 0.997 | 0.945 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 295 | 0.2 M Disodium Tartrate, 20 % PEG 3350 |