6DFA

Kaiso (ZBTB33) E535A zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9765
Spacegroup name	C 2 2 2
Unit cell lengths	44.372, 183.587, 104.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.477 - 1.908
R-factor	0.1831
Rwork	0.181
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6m
RMSD bond length	0.011
RMSD bond angle	1.125
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.477	1.980
High resolution limit [Å]	1.908	1.908
Rmerge	0.077
Rmeas	0.083
Number of reflections	33416	2981
<I/σ(I)>	10.28	1.65
Completeness [%]	98.6	89.75
Redundancy	6.8	5.4
CC(1/2)	0.999	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.2 M Disodium Tartrate, 20 % PEG 3350