6DF9
Kaiso (ZBTB33) E535Q zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 44.452, 183.142, 105.683 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.198 - 2.319 |
R-factor | 0.1767 |
Rwork | 0.172 |
R-free | 0.21780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f6m |
RMSD bond length | 0.010 |
RMSD bond angle | 1.015 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.198 | 2.400 |
High resolution limit [Å] | 2.319 | 2.320 |
Rmerge | 0.061 | |
Rmeas | 0.068 | |
Number of reflections | 18818 | 1559 |
<I/σ(I)> | 23.21 | |
Completeness [%] | 97.7 | 82.53 |
Redundancy | 11.5 | 8.4 |
CC(1/2) | 0.997 | 0.974 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 295 | 0.2 M Disodium Tartrate, 20 % PEG 3350 |