6DF9

Kaiso (ZBTB33) E535Q zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 2
Unit cell lengths	44.452, 183.142, 105.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.198 - 2.319
R-factor	0.1767
Rwork	0.172
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6m
RMSD bond length	0.010
RMSD bond angle	1.015
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.198	2.400
High resolution limit [Å]	2.319	2.320
Rmerge	0.061
Rmeas	0.068
Number of reflections	18818	1559
<I/σ(I)>	23.21
Completeness [%]	97.7	82.53
Redundancy	11.5	8.4
CC(1/2)	0.997	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.2 M Disodium Tartrate, 20 % PEG 3350