6DEY
Aspartylglucosaminuria mutant structure and function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.980, 96.560, 61.750 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.630 |
| R-factor | 0.17019 |
| Rwork | 0.169 |
| R-free | 0.19739 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.133 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.800 | 1.641 |
| High resolution limit [Å] | 1.630 | 1.600 |
| Number of reflections | 68901 | |
| <I/σ(I)> | 0 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100mM Tri, pH 7.5, 50 mM NaCl, 10 mM EDTA |
