6DEV
Human caspase-6 E35K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.385, 91.326, 86.682 |
| Unit cell angles | 90.00, 91.80, 90.00 |
Refinement procedure
| Resolution | 36.470 - 2.348 |
| R-factor | 0.2078 |
| Rwork | 0.205 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fxo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.467 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.470 | 2.430 |
| High resolution limit [Å] | 2.348 | 2.348 |
| Number of reflections | 41219 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 98.0 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100 mM sodium acetate pH 4.6, 2 M sodium chloride |
