6DEI

Structure of Dse3-Csm1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99833
Spacegroup name	P 21 21 21
Unit cell lengths	41.442, 51.235, 117.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.095 - 1.699
R-factor	0.1911
Rwork	0.189
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n4s
RMSD bond length	0.007
RMSD bond angle	0.763
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	1.730
High resolution limit [Å]	1.699	1.699
Number of reflections	28421
<I/σ(I)>	8.3
Completeness [%]	99.1	95.1
Redundancy	6.5
CC(1/2)	0.997	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M NaOAc, 0.1M HEPES pH 7.5, 22% PEG 4000