6DEH

Structure of LpnE Effector Protein from Legionella pneumophila (sp. Philadelphia)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	67.704, 84.064, 128.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.770 - 1.800
R-factor	0.1898
Rwork	0.189
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ouv
RMSD bond length	0.011
RMSD bond angle	1.071
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.083
Rmeas	0.072	1.387
Number of reflections	74365	11787
<I/σ(I)>	21.12	1.81
Completeness [%]	99.8
Redundancy	14.2
CC(1/2)		0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	20% PEG 8000, 0.2 M ammonium sulfate, 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES), pH 6.5