6DEG
Crystal structure of a DNA polymerase III subunit beta DnaN sliding clamp from Bartonella birtlesii LL-WM9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97982 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.510, 157.670, 53.540 |
Unit cell angles | 90.00, 110.81, 90.00 |
Refinement procedure
Resolution | 47.702 - 2.450 |
R-factor | 0.2189 |
Rwork | 0.215 |
R-free | 0.25720 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.702 | 47.702 | 2.510 |
High resolution limit [Å] | 2.450 | 10.960 | 2.450 |
Rmerge | 0.040 | 0.019 | 0.549 |
Rmeas | 0.046 | 0.022 | 0.636 |
Total number of observations | 114643 | ||
Number of reflections | 29973 | 334 | 2204 |
<I/σ(I)> | 19.48 | 49.01 | 2.27 |
Completeness [%] | 98.2 | 92.5 | 98.9 |
Redundancy | 3.825 | 3.527 | 3.906 |
CC(1/2) | 0.999 | 0.999 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 287 | BabiA.17987.a.B1.PS38344 at 13.7 mg/mL against JCSG+ screen condition H7 0.2 M ammonium sulfate, 0.1 M BisTris pH 5.5, 25% PEG 3350, 15% EG as cryo-protectant, crystal tracking ID 295132h7, unique puck ID zts9-10 |