6DED

Crystal structure of the C-terminal ARM domain of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 351-722

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9797423
Spacegroup name	P 1 21 1
Unit cell lengths	76.365, 76.170, 92.257
Unit cell angles	90.00, 93.12, 90.00

Refinement procedure

Resolution	35.699 - 2.204
R-factor	0.216
Rwork	0.215
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SAD model
RMSD bond length	0.003
RMSD bond angle	0.595
Data reduction software	HKL-3000 (2.3.10)
Data scaling software	HKL-3000 (2.3.10)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmeas	0.147
Rpim	0.087
Number of reflections	53032	2657
<I/σ(I)>	18	1.3
Completeness [%]	99.3	98.6
Redundancy	4.7	3.8
CC(1/2)		0.302

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	50 mM Tris, pH 7.5, 700 mM sodium potassium tartrate