6DE8
Crystal Structure of Bifunctional Enzyme FolD-Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.887, 59.253, 73.240 |
| Unit cell angles | 90.00, 100.44, 90.00 |
Refinement procedure
| Resolution | 42.770 - 2.104 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.22590 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.465 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.122 | 0.881 |
| Number of reflections | 40403 | 2008 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.4 |
| CC(1/2) | 0.651 | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M potassium iodide, 20 % (v/v) PEG 3350 |
