6DD9

Structure of mouse SYCP3, P1 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	P 1
Unit cell lengths	45.869, 52.275, 75.595
Unit cell angles	95.17, 103.70, 110.54

Refinement procedure

Resolution	72.103 - 2.300
R-factor	0.2894
Rwork	0.287
R-free	0.33100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dd8
RMSD bond length	0.008
RMSD bond angle	0.933
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.110	2.440
High resolution limit [Å]	2.300	2.300
Rmeas	0.088	0.967
Number of reflections	51353	8372
<I/σ(I)>	9.98	1.45
Completeness [%]	92.0	92.6
Redundancy	3.7	3.7
CC(1/2)	0.999	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100 mM Tris-HCl pH 8.5, 16% PEG 4000, 100-200 mM sodium acetate