6DD8

Structure of mouse SYCP3, P21 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	45.810, 49.396, 150.264
Unit cell angles	90.00, 90.80, 90.00

Refinement procedure

Resolution	46.925 - 2.600
R-factor	0.2574
Rwork	0.254
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	150.000	2.750
High resolution limit [Å]	2.600	2.600
Rmeas	0.132	1.660
Number of reflections	40144	6425
<I/σ(I)>	5.78	0.79
Completeness [%]	98.7	98.7
Redundancy	3.5	3.5
CC(1/2)	0.997	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100 mM Tris-HCl pH 8.5, 16% PEG 4000, 100-200 mM sodium acetate