6DD8
Structure of mouse SYCP3, P21 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.810, 49.396, 150.264 |
Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
Resolution | 46.925 - 2.600 |
R-factor | 0.2574 |
Rwork | 0.254 |
R-free | 0.32300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Arcimboldo |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 150.000 | 2.750 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmeas | 0.132 | 1.660 |
Number of reflections | 40144 | 6425 |
<I/σ(I)> | 5.78 | 0.79 |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.997 | 0.783 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris-HCl pH 8.5, 16% PEG 4000, 100-200 mM sodium acetate |