6DD2
Crystal structure of Selaginella moellendorffii HCT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.390, 83.749, 188.855 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.502 - 2.906 |
R-factor | 0.2537 |
Rwork | 0.249 |
R-free | 0.30660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kjs |
RMSD bond length | 0.005 |
RMSD bond angle | 0.763 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.540 | 2.960 |
High resolution limit [Å] | 2.810 | 2.810 |
Rmerge | 0.141 | 0.620 |
Rmeas | 0.715 | |
Rpim | 0.345 | |
Number of reflections | 15752 | 2270 |
<I/σ(I)> | 9 | 2.4 |
Completeness [%] | 98.3 | 99.4 |
Redundancy | 3.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 10.2 mg/mL SmHCT was used in a 2 uL protein, 1 uL reservoir solution hanging drop setup. The reservoir solution consisted of 0.1 M HEPES:NaOH, 2 M ammonium sulfate at pH 6.5. |