6DCS

Stage III sporulation protein AF (SpoIIIAF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.286
Spacegroup name	P 41 21 2
Unit cell lengths	119.365, 119.365, 40.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.659 - 2.700
R-factor	0.2431
Rwork	0.240
R-free	0.29940
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.238
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.084	0.057	0.304
Rmeas	0.091	0.062	0.345
Rpim	0.034	0.024	0.157
Total number of observations	56622
Number of reflections	8421	508	332
<I/σ(I)>	11.6
Completeness [%]	98.0	99.8	80
Redundancy	6.7	6.4	3.7
CC(1/2)		0.997	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	2M Ammonium sulfate