6DCM
The atomic resolution crystal structure of Kringle 2 variant bound with EACA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-14 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.825, 39.006, 31.316 |
Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
Resolution | 23.458 - 1.031 |
R-factor | 0.1423 |
Rwork | 0.141 |
R-free | 0.15690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i5k |
RMSD bond length | 0.005 |
RMSD bond angle | 0.858 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.460 | 1.068 |
High resolution limit [Å] | 1.031 | 1.031 |
Rmerge | 0.062 | 0.368 |
Rmeas | 0.070 | 0.447 |
Number of reflections | 31093 | |
<I/σ(I)> | 34.5 | 4.7 |
Completeness [%] | 98.8 | 99.8 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | PEG 4000, Sodium acetate, Ammonium acetate |