6DCJ
LpoA N-terminal domain from Haemophilus influenzae; monoclinic form at 1.35 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 140 |
Detector technology | CCD |
Collection date | 2003-11-12 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.988, 36.927, 95.359 |
Unit cell angles | 90.00, 108.08, 90.00 |
Refinement procedure
Resolution | 34.440 - 1.350 |
R-factor | 0.1582 |
Rwork | 0.155 |
R-free | 0.18990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p29 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.867 |
Data reduction software | d*TREK (8.0I) |
Data scaling software | d*TREK (8.0I) |
Phasing software | PHASER (2.7.2) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.500 | 34.500 | 1.400 |
High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
Rmerge | 0.047 | 0.034 | 0.262 |
Rmeas | 0.047 | ||
Total number of observations | 303668 | ||
Number of reflections | 91763 | 8441 | 6012 |
<I/σ(I)> | 13.1 | 29.8 | 2.9 |
Completeness [%] | 90.3 | 80.6 | 59.9 |
Redundancy | 3.28 | 3.16 | 2.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Protein was 10 mg/ml in 0.1% beta-mercaptoethanol, 1 mM EDTA, 0.1 mM benzamidine, 20 mM Tris-HCl pH 8.0. Precipitant contained 20% polyethylene glycol 10,000, 0.1 M HEPES pH 7.5. 2 ul protein mixed with 2 ul precipitant on silated cover slip and equilibrated over a reservoir containing 1 ml precipitant. |