6DCF
Crystal structure of a Mycobacterium smegmatis transcription initiation complex with Rifampicin-resistant RNA polymerase and bound to kanglemycin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-12-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 129.250, 160.909, 136.864 |
| Unit cell angles | 90.00, 110.75, 90.00 |
Refinement procedure
| Resolution | 43.890 - 3.450 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cce |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.570 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.162 | 1.600 |
| Number of reflections | 68062 | |
| <I/σ(I)> | 8.02 | 0.67 |
| Completeness [%] | 97.8 | 98.8 |
| Redundancy | 4.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1 M Bis-Tris, pH 6.0, 0.2 M Li-sulfate, 20% (w/v) PEG 3350, 2.5% (v/v) ethylene glycol, 1% (v/v) DMSO |
