6DBS
Fusion surface structure, function, and dynamics of gamete fusogen HAP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-11 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.071951 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.220, 117.600, 115.210 |
| Unit cell angles | 90.00, 123.79, 90.00 |
Refinement procedure
| Resolution | 48.995 - 2.602 |
| R-factor | 0.2383 |
| Rwork | 0.237 |
| R-free | 0.27570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mf1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.528 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol (V1.0) |
| Refinement software | PHENIX (V1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.095 | 3.559 |
| Number of reflections | 66071 | 4346 |
| <I/σ(I)> | 9.4 | 0.33 |
| Completeness [%] | 97.3 | 85.8 |
| Redundancy | 4.7 | 3.3 |
| CC(1/2) | 0.999 | 0.160 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | 26% PEG3350, 0.1 M HEPES, pH 7.5, 0.35 M ammonium acetate |
