6DBD

Crystal Structure of VHH R326

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-12
Detector	RAYONIX MX-300
Wavelength(s)	0.97950
Spacegroup name	P 65 2 2
Unit cell lengths	97.701, 97.701, 243.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.850 - 1.755
R-factor	0.1607
Rwork	0.160
R-free	0.18190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dba
RMSD bond length	0.012
RMSD bond angle	1.082
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.510	69.510	1.800
High resolution limit [Å]	1.755	7.850	1.760
Rmerge	0.076	0.039	0.790
Number of reflections	68308
<I/σ(I)>	19.8	37.1	4.2
Completeness [%]	98.4	96.7	93.4
Redundancy	17.5	16.2	17.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M ammonium sulfate, 0.1< sodium acetate, 22% PEG 4000